Download e-book for iPad: Alternative pre-mRNA Splicing: Theory and Protocols by Stefan Stamm, Chris Smith, Reinhard Lührmann

By Stefan Stamm, Chris Smith, Reinhard Lührmann

ISBN-10: 3527326065

ISBN-13: 9783527326068

ISBN-10: 3527636773

ISBN-13: 9783527636778

The booklet involves significant components: The first one offers a quick theoretical
introduction that offers a brief review of other splicing and cites key papers within the field for extra in-depth details. the second one half is a set of experimental protocols which are utilized in the field of different splicing.
Contents
1 Splicing within the RNA World
2 RNPs, Small RNAs, and miRNAs
3 RNA parts interested in Splicing
4 A Structural Biology viewpoint of Proteins keen on Splicing Regulation
5 The Spliceosome in Constitutive Splicing
6 using Saccharomyces cerevisiae to check the Mechanism of pre-mRNA Splicing
7 demanding situations in Plant substitute Splicing
8 replacement Splice website Selection
9 Integration of Splicing with Nuclear and mobile Events
10 Splicing and Disease
11 From Bedside to Bench: the best way to study a Splicing Mutation
12 research of universal Splicing Problems
13 Ultracentrifugation within the research and Purification of Spliceosomes Assembled In Vitro
14 Chemical Synthesis of RNA
15 RNA Interference (siRNA, shRNA)
16 Expression and Purification of Splicing Proteins
17 Detection of RNA–Protein Complexes via Electrophoretic Mobility Shift Assay
18 sensible research of enormous Exonic Sequences via Iterative In Vivo Selection
19 id of Splicing cis-Elements via an Ultra-Refined Antisense Microwalk
20 Genomic SELEX to spot RNA goals of Plant RNA-Binding Proteins
21 Quantification of other Splice Variants
22 High-Throughput research of other Splicing by way of RT-PCR
23 tracking alterations in Plant replacement Splicing Events
24 Array Analysis
25 The CLIP option to research Protein–RNA Interactions in Intact Cells and Tissues
26 RNA–Protein Crosslinking and Immunoprecipitation (CLIP) in Schizosaccharomyces pombe
27 identity of Proteins certain to RNA
28 Single-Cell Detection of Splicing occasions with Fluorescent Splicing Reporters
29 The guidance of HeLa phone Nuclear Extracts
30 In Vitro Splicing Assays
31 meeting and Isolation of Spliceosomal Complexes In Vitro
32 research of Site-Specific RNA–Protein Interactions
33 Immunoprecipitation and Pull-Down of Nuclear Proteins
34 research of Protein (-RNA) Complexes by means of (Quantitative) Mass Spectrometric Analysis
35 quick Cloning of Splicing Reporter Minigenes
36 In Vivo research of Splicing Assays
37 Coupled Promoter Splicing Systems
38 good cellphone strains with Splicing Reporters
39 Splicing issue ChIP and ChRIP: Detection ofSplicing and Splicing elements at
Genes by means of Chromatin Immunoprecipitation
40 Yeast Genetics to enquire the functionality of center Pre-mRNA Splicing Factors
41 research of HIV-1 RNA Splicing
42 In Vivo research of Plant Intron Splicing
43 amendment State-Specific Antibodies
44 research of different Splicing in Drosophila Genetic Mosaics
45 Antisense Derivatives of U7 Small Nuclear RNA as Modulators of Pre-mRNA Splicing
46 Screening for replacement Splicing Modulators
47 Use of Oligonucleotides to alter Splicing
48 altering signs to the Spliceosome
49 evaluate of Splicing proper Databases
50 research of RNA Transcripts by means of High-Throughput RNA Sequencing
51 identity of Splicing issue goal Genes through High-Throughput Sequencing
52 Bioinformatic research of Splicing Events
53 research of Pre-mRNA Secondary constructions and substitute Splicing
54 constitution Prediction for however Spliced Proteins
55 Comparative Genomics equipment for the Prediction of Small RNA-Binding websites

Show description

By Stefan Stamm, Chris Smith, Reinhard Lührmann

ISBN-10: 3527326065

ISBN-13: 9783527326068

ISBN-10: 3527636773

ISBN-13: 9783527636778

The booklet involves significant components: The first one offers a quick theoretical
introduction that offers a brief review of other splicing and cites key papers within the field for extra in-depth details. the second one half is a set of experimental protocols which are utilized in the field of different splicing.
Contents
1 Splicing within the RNA World
2 RNPs, Small RNAs, and miRNAs
3 RNA parts interested in Splicing
4 A Structural Biology viewpoint of Proteins keen on Splicing Regulation
5 The Spliceosome in Constitutive Splicing
6 using Saccharomyces cerevisiae to check the Mechanism of pre-mRNA Splicing
7 demanding situations in Plant substitute Splicing
8 replacement Splice website Selection
9 Integration of Splicing with Nuclear and mobile Events
10 Splicing and Disease
11 From Bedside to Bench: the best way to study a Splicing Mutation
12 research of universal Splicing Problems
13 Ultracentrifugation within the research and Purification of Spliceosomes Assembled In Vitro
14 Chemical Synthesis of RNA
15 RNA Interference (siRNA, shRNA)
16 Expression and Purification of Splicing Proteins
17 Detection of RNA–Protein Complexes via Electrophoretic Mobility Shift Assay
18 sensible research of enormous Exonic Sequences via Iterative In Vivo Selection
19 id of Splicing cis-Elements via an Ultra-Refined Antisense Microwalk
20 Genomic SELEX to spot RNA goals of Plant RNA-Binding Proteins
21 Quantification of other Splice Variants
22 High-Throughput research of other Splicing by way of RT-PCR
23 tracking alterations in Plant replacement Splicing Events
24 Array Analysis
25 The CLIP option to research Protein–RNA Interactions in Intact Cells and Tissues
26 RNA–Protein Crosslinking and Immunoprecipitation (CLIP) in Schizosaccharomyces pombe
27 identity of Proteins certain to RNA
28 Single-Cell Detection of Splicing occasions with Fluorescent Splicing Reporters
29 The guidance of HeLa phone Nuclear Extracts
30 In Vitro Splicing Assays
31 meeting and Isolation of Spliceosomal Complexes In Vitro
32 research of Site-Specific RNA–Protein Interactions
33 Immunoprecipitation and Pull-Down of Nuclear Proteins
34 research of Protein (-RNA) Complexes by means of (Quantitative) Mass Spectrometric Analysis
35 quick Cloning of Splicing Reporter Minigenes
36 In Vivo research of Splicing Assays
37 Coupled Promoter Splicing Systems
38 good cellphone strains with Splicing Reporters
39 Splicing issue ChIP and ChRIP: Detection ofSplicing and Splicing elements at
Genes by means of Chromatin Immunoprecipitation
40 Yeast Genetics to enquire the functionality of center Pre-mRNA Splicing Factors
41 research of HIV-1 RNA Splicing
42 In Vivo research of Plant Intron Splicing
43 amendment State-Specific Antibodies
44 research of different Splicing in Drosophila Genetic Mosaics
45 Antisense Derivatives of U7 Small Nuclear RNA as Modulators of Pre-mRNA Splicing
46 Screening for replacement Splicing Modulators
47 Use of Oligonucleotides to alter Splicing
48 altering signs to the Spliceosome
49 evaluate of Splicing proper Databases
50 research of RNA Transcripts by means of High-Throughput RNA Sequencing
51 identity of Splicing issue goal Genes through High-Throughput Sequencing
52 Bioinformatic research of Splicing Events
53 research of Pre-mRNA Secondary constructions and substitute Splicing
54 constitution Prediction for however Spliced Proteins
55 Comparative Genomics equipment for the Prediction of Small RNA-Binding websites

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Extra info for Alternative pre-mRNA Splicing: Theory and Protocols

Example text

Science, 319, 1787–1789. , and Montanini, B. (2009) Eukaryotic snoRNAs: a paradigm for gene expression flexibility. Genomics, 94, 83–88. P. (2007) Non-coding RNAs: lessons from the small nuclear and small nucleolar RNAs. Nat. Rev. Mol. , 8, 209–220. S. (2009) Small RNAs derived from snoRNAs. RNA, 15, 1233–1240. , and Stamm, S. (2010) The snoRNA MBII-52 (SNORD 115) is processed into smaller RNAs and regulates alternative splicing. Hum. Mol. , 19, 1153–1164. Kishore, S. and Stamm, S. (2006) The snoRNA HBII-52 regulates alternative splicing of the serotonin receptor 2C.

1 Introduction: The Fascination of Alternative Pre-mRNA Splicing The genetic information is stored in DNA, which is transferred from one generation to the next. During the life of a cell, this DNA information is retrieved as RNA. Whereas DNA is chemically very stable and therefore well suited to archive the genetic information, RNA is chemically more reactive, and thus unstable. Therefore, with the exception of RNA viruses, RNA does not store the genetic information but rather acts as an intermediate between DNA and proteins.

Anderson Cancer Center Department of Genetics 1515 Holcombe Blvd. Houston, TX 77030 USA List of Contributors and University of Texas Graduate School of Biomedical Sciences Genes and Development Graduate Program Houston, TX USA Akila Mayeda Fujita Health University Institute for Comprehensive Medical Science (ICMS) Division of Gene Expression Mechanism Toyoake, Aichi 470-1192 Japan Gunter Meister Universität Regensburg Fakultät für Biologie und Vorklinische Medizin Lehrstuhl für Biochemie I Universitätsstraße 31 93053 Regensburg Germany and Max-Planck-Institute of Biochemistry Center for Integrated Protein Science Munich Am Klopferspitz 18 82152 Martinsried/Munich Germany Manuel J.

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Alternative pre-mRNA Splicing: Theory and Protocols by Stefan Stamm, Chris Smith, Reinhard Lührmann


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