Read e-book online Animal Cells as Bioreactors PDF

By Terence Cartwright

ISBN-10: 0511565062

ISBN-13: 9780511565069

ISBN-10: 052110310X

ISBN-13: 9780521103107

ISBN-10: 0521412587

ISBN-13: 9780521412582

This publication covers all facets of the hot applied sciences had to flip animal cells into an appropriate and low-budget software for drug creation. This contains editing them genetically in order that they produce the precise product in excessive yield, getting them to develop reproducibly on an commercial scale, and extracting the necessary product from them. It additionally covers organic questions of safety, and the verification of the chemical and organic nature of the protein drug produced. The paintings covers the most recent advancements in all of those parts and the way all of them have to be built-in for the layout of a good biotechnological construction procedure.

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By Terence Cartwright

ISBN-10: 0511565062

ISBN-13: 9780511565069

ISBN-10: 052110310X

ISBN-13: 9780521103107

ISBN-10: 0521412587

ISBN-13: 9780521412582

This publication covers all facets of the hot applied sciences had to flip animal cells into an appropriate and low-budget software for drug creation. This contains editing them genetically in order that they produce the precise product in excessive yield, getting them to develop reproducibly on an commercial scale, and extracting the necessary product from them. It additionally covers organic questions of safety, and the verification of the chemical and organic nature of the protein drug produced. The paintings covers the most recent advancements in all of those parts and the way all of them have to be built-in for the layout of a good biotechnological construction procedure.

Show description

Read or Download Animal Cells as Bioreactors PDF

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Additional info for Animal Cells as Bioreactors

Example text

Although in some instances copy number paralleled expression levels, in others this was not the case. In particular, K-562 human myelogenous leukaemia cells produced levels of tPA mRNA that were similar to the highest producing cell line studied (a CHO derivative), although tPA production was at least 20-fold less. It was shown that about 96% of the tPA produced in these cells was secreted, so the deficiency in K-562 cells was presumed to be at the level of messenger translation (Jalanko et al, 1990).

It is generally accepted that probably all bovine populations contain BVD which is transmitted transplacentally and most commonly causes clinically inapparent infection (Roeder and Harkness, 1986). In serum production from such a population, the level of BVD in the unfiltered serum product depends heavily on the quality of the processing steps used. BVD occurs intracellularly in leucocytes and is released by cell lysis. Thus, effective centrifugation procedures to remove all leucocytes are very important, as is rapid processing to limit leucocyte lysis.

7). 6 (jug of tPA/106 cells/d when adapted to grow in serum-free medium in stirred-tank fermentors. In batch cultures, HYB F2 reached a plateau of tPA production of 100 |xg/106 cells over 6 d. Under these conditions, tPA was directly visible by HPLC in the unpurified culture supernatant. Somatic hybridization has also been used by Chenciner et al (1990) to enhance the yield of a recombinant hepatitis-B surface antigen. In this case, the hybridoma was produced from primary monkey hepatocytes and recombinant Vero cells expressing the antigen.

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Animal Cells as Bioreactors by Terence Cartwright


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